_List.AddRange(Zeile.Split(";"))

 _List.Add(Zeile.Split(";"))

Imports System
Imports System.IO
 
Public Class Form1
 
    Private Sub Form1_Load(ByVal sender As System.Object, ByVal e As _
      System.EventArgs) Handles MyBase.Load
        ' Listview-Einstellung ändern
        With ListView1
            .View = View.Details
            .GridLines = True
            .FullRowSelect = True
            .MultiSelect = False
 
            ' 10 Spalten hinzufügen
            For i As Integer = 0 To 9
                .Columns.Add("Spalte " & i.ToString("00"))
            Next
        End With
    End Sub
 
    Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As _
      System.EventArgs) Handles Button1.Click
        For Each _Zeile() As String In Me.ReadTxtToList("c:\test.txt")
            ListView1.Items.Add(New ListViewItem(_Zeile))
        Next
        ListView1.AutoResizeColumns(ColumnHeaderAutoResizeStyle.ColumnContent)
    End Sub
 
    Private Function ReadTxtToList(ByVal sFile As String) As List(Of String())
        Dim _List As New List(Of String())
        If File.Exists(sFile) Then
            For Each Zeile As String In File.ReadAllLines(sFile)
                _List.Add(Zeile.Split(";"))
            Next
        End If
        Return _List
    End Function
End Class