Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As _
  System.EventArgs) Handles Button1.Click
 
        Dim RowCount As Integer = DataGridView1.RowCount - 1
 
        DataGridView1.Rows(RowCount).Cells(1).Value = "NEIN"
        Me.DataGridView1.DataSource = ds.Tables("MeineLinks")
 
    End Sub